Research Facilities
In Vivo Isotope Kinetic Facility
The In Vivo Isotope Kinetic Facility is an integral resource for investigators whose research requires isotope kinetic techniques for the in vivo assessment of amino acid and protein metabolism in individual organs/tissues and the whole animal. This laboratory consists of a surgical room for instrumentation of animals with indwelling intravenous catheters for isotope administration, blood sampling, and parenteral nutrition and placement of post-pyloric feeding tubes for enteral nutrition. Animals are housed in metabolic cages for the assessment of whole body nitrogen balance and energy metabolism. This facility maintains state of the art instrumentation for assessment of O2 consumption, CO2 production, and respiratory quotient, total urinary nitrogen analysis by chemiluminescence, amino acid analysis by high pressure liquid chromatography, and quantitation of radiolabeled amino acids by liquid scintillation detection.
Light and Fluorescence Microscopy Facility
The Light and Fluorescence Microscopy Facility is an invaluable resource for investigators whose research requires identification of cells and sub-microscopic cellular components with a high degree of specificity and sensitivity. This facility uses state of the art immunofluorescence and histochemical staining techniques to identify cell structures and the presence of specific antigens present in tissue sections. The facility is appropriately equipped for helping investigators whose research requires processing, fixation, and sectioning of tissues for microscopic analysis. Instrumentation in this facility also supports cell differential and morphometric analysis.
Tissue Culture Facility
The Tissue Culture Facility is used to support investigators conducting in vitro experimentation of inflammatory cells such as neutrophils and alveolar and peritoneal macrophages. This resource consists of a temperature and CO2 controlled tissue incubator and a laminar flow tissue culture hood.
Non-linear Complex Systems Analysis Laboratory
Non-linear Complex Systems Analysis Laboratory is used to discover complex interactions and emergent properties of physiological systems. The approach provides a means to study systemic properties of patients and animals for evaluation and differentiation of the systemic properties of a complex nonlinear system. This resource consists of a multi-channel EEG-2110 Nihon-Kohden instrument for collection of nonlinear time series physiological signals, including EKG and EEG signals.
The University of Tennessee Graduate School of Medicine
Department of Surgery
Graduate School of Medicine
The University of Tennessee Medical Center
l924 Alcoa Highway, Box U-11
Knoxville, Tennessee 37920