Tina Anderson Richey, M.S.
Research Associate III
Small Animal Expert
Preclinical and Diagnostic Molecular Imaging Laboratory
Education
East Tennessee State University
Johnson City, TN
B.S. - Biology
University of Tennessee
Knoxville, TN
M.S. - Microbiology
Experience
July 2003-present: Research Associate III, Human Immunology and Cancer Program, University of Tennessee Graduate School of Medicine. Maintaining transgenic mice colonies including genotyping, mouse experimental work including induction of amyloidosis, injections, necropsies, and CT/SPECT and PET imaging.
August 2001-July 2003: Research Associate, Amyloid and Neurodegenerative Diseases Laboratory, University of Tennessee Medical Center. Screening of chemical libraries for inhibition of polyglutamine aggregation, anti-amyloid monoclonal antibody production.
March 1998- August 2001: Research Associate, Department of Entomology and Plant Pathology, University of Tennessee. ELISA of fescue endophyte, detection and isolation of chitinase in fescue, testing of biocontrol organisms, DNA amplification fingerprinting, gas chromatography/mass spectroscopy of plant essential oils.
Publications
Wall, J. S., T. Richey, A. Allen, R. Donnell, S. J. Kennel and A. Solomon. Quantitative microSPECT/CT imaging of early onset spontaneous AA amyloidosis in H2-L-huIL-6 Tg Balb/c mice. Comp. Med. (in press).
Solomon, A., T. Richey, C. Murphy, D. Weiss, J. Wall, G. Westermark, and P. Westermark. 2007. Amyloidogenic potential of foie gras. PNAS104: 10998-11001.
J. D. Woodward, S. J. Kennel, S. Mirzadeh, S. Dai, J. S. Wall, T. Richey, J. Avenell and A. J. Rondinone. 2007. In vivo SPECT/CT imaging and biodistribution using radioactive Cd 125mTe/ZnS nanoparticles. Nanotechnology 18.
J.S. Wall, S.J. Kennel, M. Paulus, J. Gregor, T. Richey, J. Avenell, J. Yapp, D. Townsend, D. T. Weiss, and A. Solomon. 2006. Radioimaging of Light Chain Amyloid with a Fibril-Reactive Monoclonal Antibody. J. Nuclear Med.47: 2016-2024
J.S. Wall, S. J. Kennel, M. J. Paulus, S. Gleason, J. Gregor, J. Baba, M. Schell, T. Richey, B. O’Nuallain, R. Donnell, P. N. Hawkins, D. T. Weiss and A. Solomon. 2005. Quantitative high resolution microradiographic imaging of amyloid deposits in a novel murine model of AA-amyloidosis. Amyloid: The Journal of Protein Folding Disorders 12(3):149-156
M. H. Logan, K. D. Gwinn, T. Richey, B. Maney and C. T. Falukner. 2004. An empirical assessment of epazote (Chenopdium ambrosiodes) as a flavoring agent in cooked beans. J. Ethnobiology. 24(1): 1-12.
Nivens, D., T. Anderson, J. Chambers, and D. White. 1993. Long-term monitoring of microbial biofilms using a quartz crystal microbalance. Anal. Chem.65: 65.
Nivens, D., T. Anderson, A. Tunlid, J. Smit, and D. White. 1993. Microbial adhesion of biofilm formation monitored by attenuated total reflection/Fourier transform infrared spectroscopy. J. Microbial. Meth. 17: 199.
Nivens, D., J. Sniatecki, T. Anderson, J. Chambers, and D. White. 1993. Multi-channel ATR/FT-IR spectrometer for on-line examination of microbial biofilms. Appl. Spectroscopy47: 668.
Wintenberg, K., T. Anderson, and T. Montie. 1990. Phosphorylated tyrosine in the flagellum filament protein of Pseudomonas aeruginosa. J. Bacteriol. 172: 5235.
Anderson, T ., T. Montie, M. Murphy, and V. McCarthy. 1989. Pseudomonas aeruginosa flagellar antibodies in cystic fibrosis patients. J. Clin. Microbiol. 27: 2789.
Anderson, T . and T. Montie. 1989. Flagellar antibody stimulated opsonophagocytosis of Pseudomonas aeruginosa associated with response to either a- or b-type flagella antigen. Can. J. Microbiol. 35: 890.
Montie, T. and T. Anderson. 1988. Enzyme-linked immunosorbent assay for detection of Pseudomonas aeruginosa H(flagellar) antigen. Eur. J. Clin. Microbiol. Inf. Dis. 7: 256.
Anderson, T. and T. Montie. 1987. Opsonophagocytosis of Pseudomonas aeruginosa treated with antiflagellar serum. Infect. Immun. 55: 3204.
Selected Abstracts/Presentations
T. Richey, A. Solomon, C. Murphy, D. Weiss, J. Wall, G. Westermark, and P. Westermark. Foie gras is a dietary source of amyloid. XI International Conference on Amyloidosis, November 2006, Woods Hole, MA.
Richey, T., A. Vass, R. Mackowski, and R. Tyndell. Biocidal efficacy of ozone in cooling towers. 1996 Abstracts of Annual Meeting, American Society for Microbiology, New Orleans, LA.
Anderson, T., D. Nivens, and D. White. Use of supercritical fluid derivitization/extraction to obtain polar lipid fatty acids and neutral lipids from environmental samples. 1993 Abstracts of Annual Meeting, American Society for Microbiology, Atlanta, GA.
Anderson, T., D. Nivens, and D. White. Use of Fourier transform infrared spectroscopy (FT-IR) to study the physiological status of Bacillus species. Slide presentation, 1992 Abstracts of Annual Meeting, American Society for Microbiology, New Orleans, LA.
Anderson, T., D. Nivens, and J. Chambers. Non-destructive monitoring of microbial biomass and chemical structure with quartz crystal microbalance and Fourier transforming infrared spectroscopy. Symposium, 1991 Society for Industrial Microbiology Annual Meeting, Philadelphia, PA.
Anderson, T., T. Montie, M. Murphy, and V. McCarthy. Pseudomonas aeruginosa flagella antibody in cystic fibrosis patients. 1989 Abstracts of Annual Meeting, American Society for Microbiology. New Orleans, LA.
Specific Research Techniques
Animal (Mouse) Techniques:
- Injections (iv, sc, im, ip)
- Necropsy
- Micro PET, SPECT/CT
- Anesthesia
- Transgenic colony maintenance including weaning and genotyping
DNA/Chromosomal:
- Sister chromatid exchange assays using Chinese hamster ovary cell line
- DNA Amplification fingerprinting
Analytical:
- Attenuated total reflection/Fourier transform infrared spectroscopy
- Gas chromatography/mass spectropscopy
- HPLC and HLPC-MS
Immunological:
- Enzyme-linked immunosorbent assay (development)
- Polyclonal antibody production and testing (rabbit)
- Monoclonal antibody production
- Isolation and purification of PMNs (mouse)
- Phagocytosis assays
- Tissue immunoblots
Protein Techniques:
- Gel electrophoresis
- Column chromatography
- Thin-layer chromatography
- Protein quantitation
Lipid Techniques:
- Bligh-Dyer lipid extraction
- Supercritical fluid extraction of bacterial lipid
- Analysis by GC and TLC with specific staining
- Microbial identification system (MIDI)
General Microbiology:
- Extensive experience in microbiological culture techniques and identification
The University of Tennessee Graduate School of Medicine
Preclinical and Diagnostic Molecular Imaging Laboratory
1924 Alcoa Highway
Knoxville, TN
37920-6999
Phone: 865 305 8497