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Jay Wimalasena, Ph.D. Education
Undergraduate
University of Southampton, Southampton, U.K., B.Sc., (Hons.) Medical Sciences.
Graduate
University of Colorado Medical School, Denver, Colorado, Ph.D., (Pharmacology).
Post-doctoral fellow. Endocrine Reproduction Research Branch, NICHD, NIH, Bethesda, MD1978-1980 Society Memberships
FASEB
American Society of Biological Chemistry and Molecular Biology
Endocrine Society
Society for the Study of Reproduction
American Association for Cancer Research Professional Experience
1997-Present
Professor and Director of Research, Department of OB/GYN, University of Tennessee Medical Center, Knoxville, TN.
2003
Professor ,Dept of Pathology, University of Tennessee College of Veterinary Medicine, Knoxville, TN
1992-1997
Associate Professor and Director of Research, Department of Obstetrics/Gynecology, University of Tennessee Medical Center, Knoxville, TN; Tenure, 1993
1987-1992
Assistant Professor, Department of Physiology, Courtesy Assistant Professor, Department of Pharmacology, University of Nebraska Medical Center, Omaha, NE
1986-1987
Assistant Professor, Dept of Biology, Lehigh University, Bethlehem,PA
1980-1982
Research Assistant Professor University of Tennessee Knoxville Teaching Experience
General Endocrinology to Seniors, University of Tennessee, Knoxville.
General Physiology to Juniors, Lehigh University.
Endocrinology to seniors and graduate students, Lehigh University.
Cellular Physiology to juniors and seniors, Lehigh University.
Instruction on Endocrine and Reproductive Physiology to medical students; Pharmacy and Allied Health students; and Nursing students, University of Nebraska Medical Center, per year.1987-1992.
Endocrine Pharmacology to medical and pharmacy students, University of Nebraska Medical Center 1987- 1992.
Graduate courses (600 level) in Cell Signal Transduction, Cancer Cell Biology, University of Tennessee 1992-2003.
Postdoctoral and Graduate Student Training
Ph.D. advisor to:
Korakod Indrapichate, Ph.D., 1990
Kathy Alpaugh, Ph.D., 1991, Lehigh University,
Dr. Tzu Hao Wang 1999, University of Tennessee
Dr. Shamila Ahamed, 2000, University of Tennessee
Post doctoral advisor of:
Dr. P. McKenzie, University of Tennessee Medical Center (1993-1998).
Advisor to:
Dr. Ingrid Fernando (Ph.D.Candidate)
Dr. A.V. Medeiros (M.S University of Tennessee 1998)
Teaching Interests Cancer biology, Signal transduction in Cell cycle and Apoptosis regulation, Hormonal regulatory mechanisms. Other Academic Activities
Regular reviewer for Oncogene, Cancer Research
Occasional reviewer for Journal of Biological Chemistry and Molecular Cellular Biology.
Reviewer for DOD Breast Cancer Research program
Research Support
A Molecular Approach to Gonadotropin Receptor Regulation HD 16857
Award: $250,000
Principal Investigator: J. Wimalasena
Receptor Mediated Immunotherapy of Ovarian Cancer . American Cancer Society #PDT-329
Award: $250,000
Principal Investigator: J. Wimalasena
A Molecular Approach to Gonadotropin Receptor Regulation.
HD 21325
Award: $350,000
Principal Investigator: J. Wimalasena
Effects of Hormones in Ovarian Cancer .
State of Nebraska Smoking and Allied Diseases Program
Award: $80,000
Principal Investigator: J. Wimalasena
Effects of Alcohol on Human Ovarian Cell Function
Agency: National Institute of Alcohol and Alcohol Abuse (RO1AA08328-01A1)
Award: $868,000
Principal Investigator: J. Wimalasena
Role of Gonadotropins in Ovarian Cancer
Agency: National Cancer Institute (R55 CA 53506).
Award: $100,000/year
Principal Investigator: J. Wimalasena
Regulation of Breast Cancer Cell Cycle by Estradiol
National Cancer Institute (CA 68538)
Award: $569,000
Principal Investigator: J. Wimalasena
Apoptosis and the Cell Cycle in Breast and Ovarian Cancer cells
Agency: NCI (CA84048)
Award $848,000
Principle Investigator: J. Wimalasena
Total funding of over $3.2 million by 2003
Pending Support
Apoptosis Regulation in ovarian cancer cells ( NIH 04-08)
P.I.: J. Wimalasena
US Army Medical Research program OC 030172 Apoptosis regulation in Ovarian Cancer(04-07))
US Army Medical Research program BC 0301903 Role of the Ras oncogene in Anti-apoptotic Functions
of Estradiol (04-07)
P.I. J.Wimalasena
Publications in Peer Reviewed Journals (Only)
1. Barton S, J Wimalasena and Schacter. Subcellular location of the kininogenase in the coagulating gland of guinea pig. Biochem. Pharmacol. 22:9, 1973.
2. Wagner K, MD Roper, BH Leichtling, J Wimalasena , and WD Wicks. Effects of 6- and 8- substituted analogs of adenosine 3'5' - monophosphate on phosphoenolpyruvate carboxykinase and tyrosine amino-transferase in hepatoma cell cultures. J. Biol. Chem. 250:231, 1975.
3. Leichtling BH, J Koontz, JL Su, K Wagner, MD Roper, J Wimalasena , and WD Wicks. Enzyme induction by cAMP in hybrid cells. Arch. Biochem. Biophys. 185:525, 1978.
4. Leichtling BH, YT Su, J Wimalasena , TK Harden, BB Wolfe, and WD Wicks. Molecular basis for the lack of hormonal responsiveness of adenylate cyclase in minimal deviation rat hepatoma cells, loss of specific receptors. J. Cell Physiol. 96:215, 1978.
5. Wimalasena J and WD Wicks. Protein kinase activation by cAMP analogs in H25 cells in vivo and its relation to enzyme
induction. Mol. Pharmacol. 16:449, 1979.
6. Wimalasena J , BH Leichtling, EJ Lewis, TA Langan, and WD Wicks. Coordinate regulation of adenylate cyclase, protein kinase and specific enzyme synthesis by Cholera toxin. Arch. Biochem. Biophys. 205:595, 1980.
7. Wicks WD, BH Leichtling, J Wimalasena , and J Koontz. Regulation of cAMP metabolism and specific protein synthesis in reuber hepatoma cells. Ann. N.Y. Acad. Sci. 349:195, 1980.
8. Kitabchi, AE, J Wimalasena , and J Barker. Specific receptor sites for d-a-tocopherol in purified isolate adrenocortical cell membrane. Biochem. Biophys. Res. Comm. 96:179, 1980.
9. Wimalasena J and ML Dufau. Water soluble gonadotropin receptors of the rat ovary. Endocrinology 110:1004, 1981.
10. Wimalasena J , M Davis, and AE Kitabchi. Receptors for d-a-tocopherol in human erythrocyte membranes. Biochem. Pharmacol. 31:3455, 1982.
11. Kitabchi AE and J Wimalasena . Specific receptors for d-a-tocopherol in human erythrocyte membranes. Biochem. Biophys. Acta. 684:200, 1982.
12. Wimalasena J , S Schwab, and ML Dufau. Soluble luteinizing hormone/human chorionic gonadotropin receptors of the rat ovary. Comparative studies of water and detergent-
soluble receptors. Endocrinology 113:618, 1983.
13. Kitabchi AE and J Wimalasena . Specific receptors for d-a-tocopherol in human erythrocyte membranes. Ann. N.Y. Acad. Sci. 393:300, 1982.
14. Wimalasena J , P Moore, JP Wiebe, JH Abel, and TT Chen. The porcine LH/hCG receptor. Characterization and Purification. J. Biol. Chem. 260:10689, 1985.
15. Wimalasena J , JH Abel, JP Wiebe, and TT Chen. The porcine LH/hCG receptor II: Is the purified receptor and oligomer of identical subunits? J. Biol. Chem. 261:9416, 1986.
16. Alpaugh K, K Indrapichate, J Abel, R Rimerman, and J Wimalasena . Purification and characterization of the human ovarian LH/hCG receptor and comparison of the properties of mammalian LH/hCG receptors. Biochem. Pharmacol. 40:2093-2104, 1990.
17. Saxena S, D Meehan, and J Wimalasena . Ethanol has direct effects on progesterone and estradiol production by human granulosa cells and other mammalian granulosa cells. Alcohol: Clin. Exp. Res. 14:522-527, 1990.
18. Wimalasena J , C Cavallo, and D Meehan. Human epithelial ovarian cancer cell steroid secretion and its control by gonadotropins. Gynecol. Oncol. 41:56-63, 1991.(53 citations by May 31st 03)
19. Indrapichate K, D Meehan, TA Lane, S-Y Chu, Ch V Rao, D Johnson, TT Chen, and J Wimalasena . Biological actions of monoclonal LH/hCG receptor antibodies. Biol. Reprod. 46:265-278, 1992.
20. Wimalasena, J , R Dostal, and D Meehan. Gonadotropins, estradiol and growth factors regulate epithelial ovarian cancer cell growth in culture. Gynecol. Oncol. 46:345-350, 1992.
21. Wimalasena J , D Meehan, R Dostal, and M De Silva. Selective Inhibition of LH action by Ethanol in Cultured Human Granulosa Cells. Alcohol: Clin. Exp. Res. 17:340-344, 1993.
22. Bukovsky, A, TT Chen, J Wimalasena , and MR Caudle. Luteinizing hormone receptor expression during follicular development and selection. Biol. Reprod. 48:1367-1382, 1993.
23. Wimalasena J . Ethanol has direct effects on human choriocarcinoma cell steroid hormone secretion. Alcohol: Clin. Exp. Res. 18:369-374, 1994.
24. Wimalasena J , D Meehan, R Dostal, JS Foster, M Cameron, and M Smith. Growth factors interact with estradiol and gonadotropins in the regulation of ovarian cancer cell growth and growth factors receptors. Oncol. Res. 5:325-337, 1993.(35 citations)
25. Wimalasena J , F Beams, and MR Caudle. Ethanol modulates the hormone secretory responses induced by epidermal growth factor in choriocarcinoma cells. Alcohol: Clin. Exp. Res. 18:1448-1455, 1994.
26. Bukovsky A, Caudle MR, Keenan JA, Wimalasena J , Foster JS, and Van Meter SE. Quantitative evaluation of the cell cycle related protein (pRb) and localization of Thy-1 differentiation protein and macrophages during follicular development and atresia.
Biol. Reprod. 52:776-792, 1995.
27. M Cameron, MR Caudle, W Sullivan, JJ Peluso, and Wimalasena J . The Steroidogenic and morphologic effects of Taxol on cultured ovarian cancer cells. Oncology Res. 7:145-156, 1995.
28. Bukovsky A, Caudle MR, Keenan JA, Wimalasena J , Foster JS, Upadhyaya NB, and Van Meter SE. Expression of cell cycle regulatory proteins (p53 pRb) in the human female genital tract. J. Assisted Reprod. Genetics 12:123-131, 1995.
29. Bukovsky A, JA Keenan, MR Caudle, J Wimalasena , NB Upadhyaya, and SE Van Meter. Immunohistochemical studies of the adult human ovary. Possible contribution of epithelial and stromal factors to folliculogenesis. Am. J. Reprod. Immunol. 33:323-340, 1995.
30. Maurer DR, J Wimalasena , K Indrapichate, D Puett, and MH Majercik. Cytoskeletal regulation of hCG-induced receptor clustering in transformed leydig cells. Cell. Physiol. Biochem. 5:361-370, 1995.
31. McKenzie PP, McClaran JD, Caudle MR, Fukuda A, and Wimalasena J . Alcohol inhibits EGF stimulated progesterone secretion from human granulosa cells. Alcohol: Clin. Exp. Res. 19:1382-1388, 1995.
32. Bukovsky A, Caudle MR, Keenan JA, Wimalasena J , Upadhyaya NB, and Van Meter SE. Is corpus luteum regression an immune mediated event? Localization of immune system components and LH receptors in human corpora lutea. Biol. Reprod. 53:1373-1384, 1995.
33. Foster JS and Wimalasena J . Estrogen regulates activity of cyclin dependent kinases and retinoblastoma protein phosphorylation in breast cancer cells. Mol. Endocrinol. 10:488-498, 1996.(99 citations)
34. Cameron M, Foster J, Bukovsky A, and Wimalasena J . Activation of MAPK by gonadotropins and cAMP in porcine granulosa cells. Biol. Reprod. 55:111-119, 1996(43 citations)
35. Dees C, Askari M, Foster S, Ahamed S and Wimalasena J . DDT mimics estradiol by stimulating breast cancer cells to enter the cell cycle.Molecular Carcinogenesis 18, 107-114, 1997
36. Dees C, Travis C, Foster S, Ahamed S and Wimalasena J . Dietary estrogens stimulate human breast cells to enter the cell cycle. Environment. Health Perspect, 105, Supp. 3, 633-636, 1997.
37. Takao Y., Honda T., Ueda M., Hattori N., Yamada S., Maeda M., Fujiwara H., Mori T. and Wimalasena J . Immunohistochemical localization of the LH/HCG receptor in human ovary: HCG enhances cell surface expression of LH/HCG receptor on luteinizing granulosa cell in vitro. Mol. Human Reprod. 3, 569-578, 1997.
38. Wang TH, Wang HS, Ichijo H, Giannakakou P, Foster JS, Fojo T, and Wimalasena J . Intracellular stress induced by microtubule-interfering agents activates c-Jun N-terminal kinase/stress-activated protein kinase (Jnk/Sapk) J. Biol. Chem. 273(9):4928-4936, 1998 .(140 citations)
39.McKenzie P.P., Foster J.S., House S.L., Bukovsky A., Caudle M.R. and Wimalasena J . Differential Expression of G1 Cyclins and Cyclin-Dependent Kinase-2 (Cdk2) Activity in Term Trophoblast from Human Placentae. Biol. of Reproduction 58:1283-1289, 1998.
40.Wang T-H. Pop D.M., Wang H-S., Saitoh M., Henley D.C., Ichijo H., and Wimalasena J. , Microtubule Dysfunction induced by Paclitaxel initiates Apoptosis through both c-Jun N-terminal kinase(JNK)dependent and independent pathways in Ovarian Cancer Cells . J.Biol Chem 274 8208-8216 1999 (70 citations)
41. Bukovsky A, Caudle MR, Keenan JA, Wimalasena J , McKenzie PP. Thy-1 differentiation protein and monocyte-derived cells during regeneration and aging of human placental villi. Amer J Reprod Immunol, 42, 135-152, 1999.
42. Bukovsky A, Ayala ME, Dominguez D, Keenan JA, Wimalasena J , McKenzie PP, Caudle MR. Postnatal androgenization induces premature aging of rat ovaries. Steroids.65 190-205. 2000.
43. Foster J.S., Henley D.C., Seth P., and Wimalasena J. , Multifaceted Regulation of Cell Cycle Progression by Estrogens: Regulation of Cdk inhibitors and CDC 25A Independent of Cyclin D1-Cdk4 Function
Mol.Cell.Biol 21.794-810. 2001.
44. Ahamed S., Foster J.S., and Bukovsky A, Wimalasena J. , Signal Transduction via the Ras/Erk pathway is essential for Zearalenone induced cell cycle progression Mol Carcinogenesis 30, 88-98, 2001.
45. Foster JS, Henley DC, Ahammed A, Wimalasena J. Estrogens and Cell cycle regulation in Breast Cancer. Trends Endocrinol & Metab 12, 320-327, 2001.
46. Bukovsky A, Caudle MR, Keenan JA, Upadhyaya NB, Van Meter S, Wimalasena J , Elder RF. Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells. BMC Dev. Biol 11, 2001.
47. Bukovsky A, Ayala ME, Dominguez R, Keenan JA, Wimalasena J , Caudle MR. Changes in ovarian interstitial cell hormone receptors and behavior of mesenchymal cells. Steroids 67, 277-289, 2002.
48. Bukovsky A, Cekanova M, Caudle MR, Wimalasena J , Foster JS, Keenan JA, Elder RF. Variability of Placental expression of Cyclin E, low molecular weight variants. Biol of Reproduction 67, 568-574 2002.
49. Ahmed S., Diehl J.A., Foster J.S., Wimalasena J. , Sequestration and down regulation of CKI are essential for activation of Cyclin E/Cdk2 in Zearalenone treated MCF7 breast cancer cells. Mol. Carcinogenesis .34.45-58,2002.
50. Fernando IR, Wimalasena J . Estradiol abrogates apoptosis in MCF7 breast cancer cells through PI-3K/AKT and Erk/RSK pathways (In Press).
51. Foster JS,Nakayama K, Wimalasena J , Estrogens regulate p27Kip1 in breast cancer cells through SKP2 dependent and independent mechanisms.(In Press).
52.Bukovsky A,Cekanova M, Caudle MR, Wimalasena J , Foster JS,Henley DC,Elder RF Expression and localization of estogen receptor alpha protein in normal and abnormal term placentae and stimulation of trophoblast differentiation by estradiol Rep Biol and Endocrinol 2003 1,13.
53. Placental expression of estrogen receptor beta and its hormone binding variant - comparison with estrogen receptor alpha and a role for estrogen receptors in asymmetric division and differentiation of estrogen-dependent cells. Bukovsky A, Caudle MR, Cekanova M, Fernando RI, Wimalasena J , Foster JS, Henley DC, Elder RF. Reprod Biol Endocrinol 2003 Apr 15;1(1):36
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