Human Immunology and Cancer Program

Amyloid Researchers

Stephen J. Kennel, Ph.D.
Associate Professor

Education

University of California, San Diego, CA - 1971
Ph.D. Chemistry

University of California, San Diego, CA - 1968
M.S. Chemistry

University of Illinois - 1967
B.S. Chemistry

Positions and Honors

2005-present    
Associate Professor, University of Tennessee, Graduate School of Medicine

1998-2005
Distinguished Senior Staff Scientist, Oak Ridge National Laboratory

1981-1998                    
Senior Staff Scientist, Oak Ridge National Laboratory

1974-1976                    
Associate, Department of Immunopathology, Scripps Clinic and Research Foundation

1973-1974                    
Assistant, Department of Experimental Pathology, Scripps Clinic and Research Foundation

1971-1973                    
Research Fellow, Department of Experimental Pathology, Scripps Clinic and Research Foundation

1967-1971                    
USPHS Graduate Trainee Fellow, University of California

1966                            
Research Assistant, Northern Regional Laboratory

Selected peer-reviewed publications (Selected from >150)

Kennel, S.J., M. Stabin, J.C.Roeske, L.J. Foote, P.K. Lankford, P. Terzaghi-Howe, H. Patterson, J. Barkenbus, D.M. Popp, R. Boll, and S. Mirzadeh.  Radiotoxicity of 213Bi Bound to Membranes of Monolayer and Spheroid Cultures of Tumor Cells. Rad. Res., 151, 244-256,1999.

Kennel, S.J., M. Stabin, M. Brechbiel, and S. Mirzadeh. Treatment of lung tumor colonies with 90Y targeted to blood vessels-Comparison with the α-emitter  213Bi. Nucl. Med. Biol., 26,149-157,1999.

Kennel S.J., P.K. Lankford, L.J. Foote, and I.A. Davis.  Monoclonal Antibody to Rat CD63 Detects Different Molecular forms in Rat Tissues. Hybridoma, 17,509-515, 1998.

Davis, I.A. and S.J. Kennel Radioimmunotherapy using vascular targeted 213Bi: The role of TNF-α in the development of pulmonary fibrosis (Clinical Cancer Research, 5, 3160s-3194s, 1999.

Cagle,D.W., S.J. Kennel, S. Mirzadeh, J.M. Alford, and L.J. Wilson,. In Vivo Studies of Fullerene-Based Materials Employing Endohedral Metallofullerene Radiotracers. PNAS, 96, 5182-5187, 1999.

Hurst, G. B., M.V. Buchanan,  L.J. Foote, and S. J. Kennel.  Analysis for TNF-α using solid-phase capture with radiolabel and MALDI-MS detection Anal. Chem., 71, 4727-4733, 1999.

Kennel, S. J., T. K. Lankford, L. J. Foote, I. A. Davis, R. A. Boll and S. Mirzadeh.  Combination Vascular Targeted and Tumor Targeted Radioimmunotherapy. Cancer Biotherapy and Radiopharmaceuticals, 14, 371-379, 1999.

Paulus, M. J., et. al High Resolution X-ray Computed Tomography: An Emerging Tool for Small Animal Cancer Research. Neoplasia 2, 62-70, 2000.

Kennel, S. J., S. Mirzadeh,, G. Hurst, L. J. Foote, T. K. Lankford, K. A. Glowienka, L. L. Chappell, J. Kelso, S. M. Davern, A. Safavey, and M. W. Brechbiel.  Labeling and distribution of linear peptides identified using in vivo phage display selection for tumors. Nucl. Med Biol. 27, 815-825, 2000.

Kennel, S. J., I. A. Davis, J. Branning, H-J. Pan, G. W. Kabalka, and M. J. Paulus.  High Resolution Computed Tomography and MRI for Monitoring Lung Tumor Growth in Mice Undergoing Radioimmunotherapy: Correlation with Histology. J Med Phys. 27, 1101-1107, 2000.

Kennel, S. J., M. W. Brechbiel, M. Deal, E. Dadachova, T. K. Lankford, I. A. Davis, M. Stabin and S. Mirzadeh.  Evaluation of 225Ac for Vascular Targeted Radioimmunotherapy of Lung Tumors.  Cancer Biotherapy and Radiopharmaceuticals 15, 235-244, 2000.

Kennel, S. J., S. Mirzadeh, W. C. Eckleman, T. A. Waldmann, K. Gharmestani, A Yordanov, M. Stabin and M. W. Brechbiel. Vascular Targeted Radioimmunotherapy with the α-Emitter 211At.  Rad Research 157, 633-641, 2002.

Kennel, S. J., M. W. Brechbiel, D Milnec, J. Schlom and S. Mirzadeh.  Actinium-225 conjugates of MAb CC49 and ∆CH2CC49: Therapy of LS174T tumors in nude mice.  Cancer Biotherapy and Radiopharaceuticals 17, 219-231, 2002.

Kennel, S. J., T. Lankford, S. Davern, L. Foote, K. Taniguchi, I. Ohizumi, Y. Tsutsumi, S. Nakagawa T. Mayumi and     S. Mirzadeh.  Therapy of rat tracheal carcinoma IC-12 in SCID mice: Vascular targeting with 213Bi-MAb TES-23.   European J.Cancer, 38, 1278-1287, 2002.

Subramanian A., P. I. Olden, S. J. Kennel, K. B. Jacobson, R. J. Warmack, T. Thundat and M. J. Doktycz. Glucose biosensing using an enzyme-coated microcantilever. Applied Physics Letters 81, 385-387, 2002.

Davern S. M., P. K. Lankford, L. J. Foote and S. J. Kennel.  Monoclonal antibodies to CD-44 epitopes on mouse endothelium. Hybridoma and Hybridomics 21 , 339-349, 2002.

Akabani G., Kennel S. J. and M. R. Zalutsky.  Microdosimetric analysis of alpha particle emitting targeted radiotherapeutics using histological images. J. Nucl. Med. 44. 792-805, 2003.

Jamasbi, R. J., Stoner, G. D., Foote, L.J., Lankford, T. K. Davern, S. and S. J. Kennel.  A monoclonal antibody to a carbohydrate epitope expressed on glycolipid and on α3β1 integrin on human esophageal carcinoma. Hybridoma and Hybridomics 22, 367-376, 2004.

DeNardo G. L., Kennel S. J., Siegel J. A. and S. J. DeNardo Radiometals as Payloads for Radioimmunotherapy for Lymphoma. Clin Lymphoma 5, S5-S10, 2004.

Kennel, S. J., Lankford, T., Foote, L., Wall, M. and S. Davern.  Phage display selection of scFv to murine endothelial cell membranes. Hybridoma and Hybridomics 23. 205-211, 2004.

Buchanan M. V., Larimer F. W., Wiley H. S., Kennel S. J., Squier T. J., Ramsey J. M., Rodland K. D., Hurst G. B., Smith R. D., Xu Y., Dixon D., Doktycz M. J., Colson S., Gesteland R., Giometti C., Young M., and M. Giddings.  Genomes to life “center for Molecular and cellular systems”: A research program for the identification and characterization of protein complexes. OMICS 6, 287-303, 2002.

Davern S. M., Foote L. J., Lankford P. K., Macy S. D., Wall M. D. and S. J. Kennel. Identification of an anti-laminin-1 scFv that preferentially homes to vascular solid tumors. Cancer Biotherapy and Radiophamaceuticals, 20, 524-533, 2005.

Wall  J. S., Kennel S. J., Paulus M. J., Gleason S., Gregor J., Baba J., Schell M., Ramsey T., O’Nullian B., Donnell R., Hawkins P. N., Weiss D., and A. Solomon.  Quantitative High-resolution microautoradiographic imaging of amyloid deposit in a novel murine model of AA-amyloidosis.  Amyloid: The Journal of Protein Folding Disorders, 12, 149-156 (2005).

Huang B. C., Foote L. J., Lankford P. K., Davern S. M., McKewon, C. and S. J. Kennel.  A diabody that dissociates to monomer forms at low concentration: Effects on binding activity and tumor targeting.  Biochem and Biophys Res Comm 327, 999-1005 (2005).

Kennel S.J.,Lankford, T, Garland M, Sunderberg, J.P. and Mirzadeh, S.  Boidistribution of 225Ra citrate in mice: retention of daughter radioisotopes in bone Nuc Med Biol 32, 859-867 (2005).

Gregor J., Gleason S., Kennel S., Paulus M., Benson T., and Wall J.  Approximate Volumetric System Models for MicroSPECT. In press Van Berkel G. J., Ford M. J., Doktycz M. J., and S. J. Kennel.  Evaluation of a surface-sampling probe electrospray mass spectrometry system for the analysis of surface-deposited and affinity-captured proteins.  Rapid Comm in Mass Spectrometry 20, 1-9, 2006.
Huang B-C, Davern S and S. J. Kennel.  Mono and bivalent binding of a scFv and covalent diabody to murine laminin-1 using radioiodinated proteins and SPR measurements: Effects on tissue retention in vivo. J Immunol. Methods 313, 149-160 (2006).

Wall J.S., Paulus M.J., Gleason S., Gregor J., Solomon A., and Kennel S. J. Micro-Imaging of amyloid in mice. Methods in Enzylogy 412 [11] 161-182 (2006).

Wall J. S., Kennel S.J., Paulus M., Gregor J., Richey T., Avenell J., Yap J., Townsend D., Weiss D., and Solomon A.  Radioimaging of AL amyloid using a fibril-reative monoclonal antibody.  J. Nucl. Med in press.

Lee, T.H., Seng, S.,Kennel, S. J., Karsenty Avraham, H., and Avraham, S.  Integrin regulationby VEGF in human brain microvascular endothelial cells: role of alpha 6 beta 1 integrin in angiogenesis. J. Biol Chem (in press)

O'Nuallain B., Allen A., Kennel S. J., Weiss D., Solomon, A. and  Wall, J. S.  Localization of a Conformational Epitope Common to Non-Native and Fibrillar Immunoglobulin Light Chains. Biochemistry ( in Press)

Research Support

 Ongoing Research Support

ORNL subcontract --Branden Enterprises                     
To support DOE projects on Genomes to Life and Nanochemistry for drug and radioisotope delivery
Role:  PI (20% effort)

No number (S. Kennel) UTGSM                                            
1/30/06-1/30/07                                              
MM1.0
Physicians’ Medical Education and Research Foundation - $10,000
In vivo imaging of Gleevec-induced apoptosis in
Mice with proliferating myelosarcoma

No Number (A. Solomon) UTGSM   
08/06- 08/09                                       
MM1.2
ELAN Pharmaceuticals  - $190,000
Diagnostic and therapeutic potential of AA Amyloid-reactive monoclonal antibodies

Test ELAN monoclonal antibodies for use in AA-Amyloid diagnosis and therapy

No Number (B. O’Nuallain)     UTGSM                                  
08/06-08/09                                        
MM0.6
Baxter AG/Biosciences - $424,000
Diagnostic and Therapeutic Potential of Naturally occurring Amyloid Fibril- and
Aβ-reactive IgG antibodies contained in human plasma and immune globulin (IGIV) products

Test IVIG antibodies for amyloid fibril reactivity in vitro

Pending

PA-01-024 (J. Wall)    UTGSM                                              
03/2002-03/2007                                            
MM2.4
NIH - (extension pending)   $886,000
High Resolution SPECT/CT Imaging of Systemic AA-Amylodoisis in Mice

Develop co-registered microSPECT/CT at high resolution for evaluation of extent of disease.